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CSNK2A1 Polyclonal antibody
CSNK2A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10992-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, PC-3 cells, K-562 cells, NIH/3T3 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 25 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
| IP | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
10992-1-AP targets CSNK2A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CSNK2A1 fusion protein Ag1452 |
| Full Name | casein kinase 2, alpha 1 polypeptide |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC011668 |
| Gene Symbol | CSNK2A1 |
| Gene ID (NCBI) | 1457 |
| RRID | AB_2245531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IHC protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IF protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IP protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy | ||
Nucleic Acids Res CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling
| ||
Nucleic Acids Res CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication.
| ||
Nucleic Acids Res ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters. | ||
Redox Biol Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury. | ||
Cancer Lett CSNK2A1 confers gemcitabine resistance to pancreatic ductal adenocarcinoma via inducing autophagy |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Janeen (Verified Customer) (07-22-2021) | There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.
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