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Placental lactogen Polyclonal antibody

Placental lactogen Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16326-1-AP

Synonyms

CSH2, hCS B, CSH1, CSB, CS 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Placental lactogen Polyclonal antibody (16326-1-AP)

    WB analysis using 16326-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    WB analysis of human placenta using 16326-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    WB analysis of human colon using 16326-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16326-1-AP (Placental lactogen antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    IHC staining of human placenta using 16326-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    IHC staining of human placenta using 16326-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    IHC staining of human placenta using 16326-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    IHC staining of human placenta using 16326-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16326-1-AP (Placental lactogen antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    IF Staining of human placenta using 16326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Placental lactogen antibody (16326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Placental lactogen Polyclonal antibody (16326-1-AP)

    IF Staining of human placenta using 16326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Placental lactogen antibody (16326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue, human colon tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16326-1-AP targets Placental lactogen in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Placental lactogen fusion protein Ag9412
Full Name chorionic somatomammotropin hormone 2
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC022044
Gene Symbol CSH2
Gene ID (NCBI) 1443
RRIDAB_2083883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomammotropin (CSH1, CSH2 and CSH-L) genes. Placental lactogens (CSH1, CSH2) are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus.

Protocols

Product Specific Protocols
WB protocol for Placental lactogen antibody 16326-1-APDownload protocol
IHC protocol for Placental lactogen antibody 16326-1-APDownload protocol
IF protocol for Placental lactogen antibody 16326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

J Vet Med Sci

Localization of peptide hormones in the placentas of Bryde's (Balaenoptera brydei), sei (B. borealis), and common minke (B. acutorostrata) whales

Authors - Risa Aoki
View Article