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CPEB3 Polyclonal antibody

CPEB3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 12669-1-AP

Synonyms

hCPEB 3, Cytoplasmic polyadenylation element-binding protein 3, CPE-BP3, CPE-binding protein 3, CPE BP3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CPEB3 Polyclonal antibody (12669-1-AP)

    WB analysis using 12669-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12669-1-AP (CPEB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPEB3 Polyclonal antibody (12669-1-AP)

    IHC staining of mouse brain using 12669-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12669-1-AP (CPEB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPEB3 Polyclonal antibody (12669-1-AP)

    IHC staining of mouse brain using 12669-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12669-1-AP (CPEB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CPEB3 Polyclonal antibody (12669-1-AP)

    IF Staining of mouse brain using 12669-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CPEB3 Polyclonal antibody (12669-1-AP)

    IF Staining of mouse brain using 12669-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CPEB3 Polyclonal antibody (12669-1-AP)

    IF Staining of mouse brain using 12669-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CPEB3 Polyclonal antibody (12669-1-AP)

    IF Staining of mouse brain using 12669-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12669-1-AP targets CPEB3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPEB3 fusion protein Ag3351
Full Name cytoplasmic polyadenylation element binding protein 3
Calculated Molecular Weight 697 aa, 76 kDa
Observed Molecular Weight 76-80 kDa
GenBank Accession NumberBC036444
Gene Symbol CPEB3
Gene ID (NCBI) 22849
RRIDAB_2877871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPEB3, the cytoplasmic polyadenylation element-binding protein 3, contains a Q/N-rich domain at its N-termina which is required for the aggregation of CPEB3. The expression of CPEB3 is downregulated in cervical cancer and hepatocellular carcinoma compared with normal tissue. It has been reported that the aggregation and activity of CPEB3 is controlled by SUMOylation. CPEB3 is SUMOylated in hippocampal neurons in the basal state while converted into an active form which is associated with a decrease of SUMOylation and an increase of aggregation. CPEB3 is expressed in cytoplasma in unstimulated neurons but translocates to the nucleus following neuronal stimulation.

Protocols

Product Specific Protocols
WB protocol for CPEB3 antibody 12669-1-APDownload protocol
IHC protocol for CPEB3 antibody 12669-1-APDownload protocol
IF protocol for CPEB3 antibody 12669-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pharmacology

MiR-224 Executes a Tumor Accelerative Role during Hepatocellular Carcinoma Malignancy by Targeting Cytoplasmic Polyadenylation Element-Binding Protein 3.

Authors - Ke Miao
View Article