COQ5 Polyclonal antibody

COQ5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17453-1-AP

Synonyms

COQ5



Tested Applications

Positive WB detected inmouse heart tissue, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman heart tissue, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17453-1-AP targets COQ5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ5 fusion protein Ag10203
Full Name coenzyme Q5 homolog, methyltransferase (S. cerevisiae)
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 34.7 kDa
GenBank Accession NumberBC107874
Gene Symbol COQ5
Gene ID (NCBI) 84274
RRIDAB_10547974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COQ5 antibody 17453-1-APDownload protocol
IHC protocol for COQ5 antibody 17453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Exp Med

Implication of folate deficiency in CYP2U1 loss of function.

Authors - Claire Pujol
mouseWB

EMBO Mol Med

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Authors - Agustín Hidalgo-Gutiérrez
mouseWB

Redox Biol

The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.

Authors - Pilar González-García
humanWB

J Am Soc Nephrol

ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment.

Authors - Eugen Widmeier
mouseWB

EMBO Mol Med

The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene.

Authors - Marta Luna-Sánchez
mouseWB

Sci Rep

Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.

Authors - María Rodríguez-Hidalgo