Tested Applications
| Positive WB detected in | HEK-293 cells, Raji cells, HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16485-1-AP targets CDC25C in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9607 Product name: Recombinant human CDC25C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-279 aa of BC019089 Sequence: MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKCCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQ Predict reactive species |
| Full Name | cell division cycle 25 homolog C (S. pombe) |
| Calculated Molecular Weight | 473 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC019089 |
| Gene Symbol | CDC25C |
| Gene ID (NCBI) | 995 |
| RRID | AB_2291330 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30307 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C(cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells(PMID:10330186). It may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop(PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa(PMID:18296513). CDC25C has some isoforms with a molecular mass of 46-53 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDC25C antibody 16485-1-AP | Download protocol |
| IP protocol for CDC25C antibody 16485-1-AP | Download protocol |
| WB protocol for CDC25C antibody 16485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDC25C Polyclonal antibody (Cat# 16485-1-AP) has accumulated 43 citations published in journals including Nature, Nature Communications, Cell Death & Disease, Acta Pharmaceutica Sinica B, Journal of Experimental & Clinical Cancer Research, and Phytomedicine. Associated research fields encompass cancer biology across multiple tumor types (lung, liver, breast, ovarian, gastric, pancreatic), cell cycle regulation, DNA damage response, drug resistance mechanisms, and natural product pharmacology.
| Species | Application | Title |
|---|---|---|
Nat Commun A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. | ||
Nat Commun m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response | ||
Acta Pharm Sin B DHL-11, a novel prieurianin-type limonoid isolated from Munronia henryi, targeting IMPDH2 to inhibit triple-negative breast cancer. | ||
J Exp Clin Cancer Res 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase. |
