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CDC25C Polyclonal antibody

CDC25C Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC

Conjugate

Unconjugated

Cat no : 16485-1-AP

Synonyms

CDC25, CDC25C, M phase inducer phosphatase 3

Validation Data Gallery

Applications:
  • WB analysis using 16485-1-AP

    WB analysis using 16485-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16485-1-AP

    WB analysis of HeLa using 16485-1-AP

    WB result of CDC25C antibody (16485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC25C transfected HeLa cells.

  • WB analysis of K-562 using 16485-1-AP

    WB analysis of K-562 using 16485-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 16485-1-AP

    WB analysis of Raji using 16485-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 16485-1-AP

    WB analysis of HepG2 using 16485-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 16485-1-AP

    IP experiment of K-562 using 16485-1-AP

    IP Result of anti-CDC25C (IP:16485-1-AP, 4ug; Detection:16485-1-AP 1:1000) with K-562 cells lysate 3600ug.

  • IHC staining of human colon cancer using 16485-1-AP

    IHC staining of human colon cancer using 16485-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16485-1-AP

    IHC staining of human colon cancer using 16485-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16485-1-AP

    IHC staining of human liver cancer using 16485-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16485-1-AP

    IHC staining of human liver cancer using 16485-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 16485-1-AP

    FC experiment of K-562 using 16485-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (16485-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, Raji cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16485-1-AP targets CDC25C in WB, IP, IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC25C fusion protein Ag9607
Full Name cell division cycle 25 homolog C (S. pombe)
Calculated molecular weight 473 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC019089
Gene symbol CDC25C
Gene ID (NCBI) 995
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C(cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells(PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop(PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa(PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Protocols

Product Specific Protocols
WB protocol for CDC25C antibody 16485-1-APDownload protocol
IHC protocol for CDC25C antibody 16485-1-APDownload protocol
IP protocol for CDC25C antibody 16485-1-APDownload protocol
FC protocol for CDC25C antibody 16485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

Cordycepin suppresses cell proliferation and migration by targeting CLEC2 in human gastric cancer cells via Akt signaling pathway.

Authors - Ying Wang
View Article
humanWB

Cell Cycle

A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.

Authors - Yanan Wang
View Article
humanWB

Cell Cycle

PMID: 31599189

Authors - Yonglong Zhang
View Article
humanWB

Laryngoscope Investig Otolaryngol

Characteristics and clinical significance of polyploid giant cancer cells in laryngeal carcinoma.

Authors - Hui-Ting Liu
View Article
humanWB

Med Sci Monit

PMID: 29519997

Authors - Shasha Liu
View Article
humanWB

Pathol Res Pract

CyclinB1 deubiquitination by USP14 regulates cell cycle progression in breast cancer.

Authors - Bing Liu
View Article