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CDC25B Polyclonal antibody

CDC25B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10644-1-AP

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CDC25B Polyclonal antibody (10644-1-AP)

    WB analysis of mouse liver using 10644-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CDC25B Polyclonal antibody (10644-1-AP)

    WB analysis of mouse liver using 10644-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CDC25B Polyclonal antibody (10644-1-AP)

    WB analysis of Raji using 10644-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CDC25B Polyclonal antibody (10644-1-AP)

    WB analysis of Jurkat using 10644-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CDC25B Polyclonal antibody (10644-1-AP)

    WB analysis of mouse brain using 10644-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CDC25B Polyclonal antibody (10644-1-AP)

    WB analysis of mouse brain using 10644-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CDC25B Polyclonal antibody (10644-1-AP)

    WB analysis of Raji using 10644-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using CDC25B Polyclonal antibody (10644-1-AP)

    IP experiment of mouse liver using 10644-1-AP

    IP result of anti-CDC25B (IP:10644-1-AP, 4ug; Detection:10644-1-AP 1:300) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CDC25B Polyclonal antibody (10644-1-AP)

    IHC staining of human lymphoma using 10644-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 10644-1-AP (CDC25B antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10644-1-AP targets CDC25B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC25B fusion protein Ag0994
Full Name cell division cycle 25 homolog B (S. pombe)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
RRIDAB_2075439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

Protocols

Product Specific Protocols
WB protocol for CDC25B antibody 10644-1-APDownload protocol
IHC protocol for CDC25B antibody 10644-1-APDownload protocol
IP protocol for CDC25B antibody 10644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IF

BMC Dev Biol

Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.

Authors - Santanu De
View Article
mouseIF

BMC Dev Biol

YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.

Authors - Alaa A Eisa
View Article