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CD207 Polyclonal antibody

CD207 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11841-1-AP

Synonyms

Langerin, C-type lectin domain family 4 member K, CLEC4K, CD207 molecule, langerin

Validation Data Gallery

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  • Western Blot (WB) analysis of human skin tissue using Langerin Polyclonal antibody (11841-1-AP)

    WB analysis of human skin using 11841-1-AP

    human skin tissue were subjected to SDS PAGE followed by western blot with 11841-1-AP (Langerin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using Langerin Polyclonal antibody (11841-1-AP)

    WB analysis of mouse skin using 11841-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11841-1-AP (Langerin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Langerin Polyclonal antibody (11841-1-AP)

    IHC staining of human skin cancer using 11841-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11841-1-AP (Langerin antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Langerin Polyclonal antibody (11841-1-AP)

    IHC staining of human skin cancer using 11841-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11841-1-AP (Langerin antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman skin tissue, mouse skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:3000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11841-1-AP targets CD207 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD207 fusion protein Ag2479
Full Name CD207 molecule, langerin
Calculated Molecular Weight 328 aa, 37 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC022278
Gene Symbol CD207
Gene ID (NCBI) 50489
RRIDAB_2074314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CD207 antibody 11841-1-APDownload protocol
IHC protocol for CD207 antibody 11841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols