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CD2 Monoclonal antibody

CD2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A10B2

Cat no : 60005-1-Ig

Synonyms

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD2 Monoclonal antibody (60005-1-Ig)

    WB analysis of Jurkat using 60005-1-Ig

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of Jurkat cells using CD2 Monoclonal antibody (60005-1-Ig)

    WB analysis of Jurkat using 60005-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using CD2 Monoclonal antibody (60005-1-Ig)

    WB analysis of human spleen using 60005-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Monoclonal antibody (60005-1-Ig)

    IHC staining of human tonsillitis using 60005-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Monoclonal antibody (60005-1-Ig)

    IHC staining of human tonsillitis using 60005-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

60005-1-Ig targets CD2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, sheep
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD2 fusion protein Ag0423
Full Name CD2 molecule
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
RRIDAB_2881350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
WB protocol for CD2 antibody 60005-1-IgDownload protocol
IHC protocol for CD2 antibody 60005-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,sheepIF

Exp Hematol

Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells

Authors - Tomoyuki Abe
View Article