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CCT8 Monoclonal antibody
CCT8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1A7C3
Cat no : 67539-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 2 publications below |
IP | See 1 publications below |
Product Information
67539-1-Ig targets CCT8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CCT8 fusion protein Ag30082 |
Full Name | chaperonin containing TCP1, subunit 8 (theta) |
Calculated Molecular Weight | 548 aa, 60 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC012584 |
Gene Symbol | CCT8 |
Gene ID (NCBI) | 10694 |
RRID | AB_2882758 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CCT8 antibody 67539-1-Ig | Download protocol |
IHC protocol for CCT8 antibody 67539-1-Ig | Download protocol |
IF protocol for CCT8 antibody 67539-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Medicine (Baltimore) Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study |