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  • KD/KO Validated

Cyclin B2 Polyclonal antibody

Cyclin B2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21644-1-AP

Synonyms

CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299



Tested Applications

Positive WB detected inA431 cells, DU 145 cells, rat testis tissue, HeLa cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21644-1-AP targets Cyclin B2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin B2 fusion protein Ag16329
Full Name cyclin B2
Calculated Molecular Weight 398 aa, 45 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC105086
Gene Symbol CCNB2
Gene ID (NCBI) 9133
RRIDAB_10755304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status. Cyclin B2 can be detected double bands (PMID:1651338).

Protocols

Product Specific Protocols
WB protocol for Cyclin B2 antibody 21644-1-APDownload protocol
IHC protocol for Cyclin B2 antibody 21644-1-APDownload protocol
IP protocol for Cyclin B2 antibody 21644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling

Authors - Chaojie Chen
humanWB,IP

EMBO Rep

Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.

Authors - Sikai Liu
  • KD Validated
humanWB

Oxid Med Cell Longev

Therapy-resistant and -sensitive lncRNAs, SNHG1 and UBL7-AS1 promote glioblastoma cell proliferation.

Authors - Mei Cao
humanIHC

Elife

Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.

Authors - Xiao-Tong Lin
humanWB

Oncotarget

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Authors - Lifei Fan
humanWB

Ecotoxicol Environ Saf

Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity.

Authors - Liling Su