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Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23821-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    WB analysis of HeLa using 23821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    WB analysis of HeLa using 23821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of UV treated Hela using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    WB analysis of UV treated Hela using 23821-1-AP

    UV treated Hela cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of UV treated Hela using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    WB analysis of UV treated Hela using 23821-1-AP

    UV treated Hela cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    WB analysis of HeLa using 23821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    IHC staining of human heart using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    IHC staining of human heart using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Caspase 9/p35/p10 Polyclonal antibody (23821-1-AP)

    IF Staining of HUVEC using 23821-1-AP

    Immunofluorescent analysis of HUVEC cells using 23821-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHeLa cells,
Positive IHC detected inhuman pancreas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23821-1-AP is Caspase 9/p35/p10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 9/p35/p10 fusion protein Ag20813
Full Name caspase 9, apoptosis-related cysteine peptidase
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002452
Gene Symbol Caspase 9
Gene ID (NCBI) 842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade

Publications

SpeciesApplicationTitle
mouseWB

J Cell Physiol

Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats.

Authors - Chao Fu
View Article