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  • Featured Product
  • KD/KO Validated

Beta Galactosidase Monoclonal antibody

Beta Galactosidase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4F4F4

Cat no : 66586-1-Ig

Synonyms

Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    WB analysis using 66586-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of A549 cells using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    WB analysis of A549 using 66586-1-Ig

    WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    IHC staining of human kidney using 66586-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    IHC staining of human kidney using 66586-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Beta Galactosidase Monoclonal antibody (66586-1-Ig)

    IF Staining of HepG2 using 66586-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66586-1-Ig (Beta galactosidase antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66586-1-Ig targets Beta Galactosidase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitysheep
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Beta Galactosidase fusion protein Ag8069
Full Name galactosidase, beta 1
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 64-66 kDa, 76-85 kDa
GenBank Accession NumberBC007493
Gene Symbol GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_2881946
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.

Protocols

Product Specific Protocols
WB protocol for Beta Galactosidase antibody 66586-1-IgDownload protocol
IHC protocol for Beta Galactosidase antibody 66586-1-IgDownload protocol
IF protocol for Beta Galactosidase antibody 66586-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepIF

Antioxidants (Basel)

Effects of Fisetin Treatment on Cellular Senescence of Various Tissues and Organs of Old Sheep

Authors - Charles A Huard
View Article