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BUD13 Polyclonal antibody

BUD13 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20163-1-AP

Synonyms

BUD13 homolog

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse testis tissue using BUD13 Polyclonal antibody (20163-1-AP)

    IHC staining of mouse testis using 20163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BUD13 Polyclonal antibody (20163-1-AP)

    IHC staining of mouse testis using 20163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using BUD13 Polyclonal antibody (20163-1-AP)

    IF Staining of A431 using 20163-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using BUD13 antibody (20163-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20163-1-AP targets BUD13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BUD13 fusion protein Ag13665
Full Name BUD13 homolog (S. cerevisiae)
Calculated Molecular Weight 619 aa, 71 kDa
Observed Molecular Weight 71-85 kDa
GenBank Accession NumberBC006350
Gene Symbol BUD13
Gene ID (NCBI) 84811
RRIDAB_2878650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BUD13 homolog (BUD13) is a component of the retention and splicing (RES) complex, previously identified in yeast as a splicing factor affecting nuclear pre-mRNA retention. As an RNA binding protein (RBP), BUD13 expression is associated with poor prognosis and may be involved in the development and progression of hepatocellular carcinoma (PMID: 37605593).

Protocols

Product Specific Protocols
IHC protocol for BUD13 antibody 20163-1-APDownload protocol
IF protocol for BUD13 antibody 20163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Exp Med

Somatic gain-of-function mutations in BUD13 promote oncogenesis by disrupting Fbw7 function

Authors - Jianfeng Chen
View Article