Filter: AllWBIPIHCIF/ICC

WB analysis using 11989-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11989-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11989-1-AP
WB result of CD147 antibody (11989-1-AP; 1:100000; room temperature for 1.5 hours) with wild-type and CD147 knockout Jurkat cells.
WB analysis of mouse heart using 11989-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11989-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11989-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11989-1-AP (CD147 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11989-1-AP
IP result of anti-CD147 (IP:11989-1-AP, 4ug; Detection:11989-1-AP 1:10000) with HEK-293 cells lysate 1200 ug.
IHC staining of human stomach cancer using 11989-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 11989-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11989-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11989-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11989-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11989-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11989-1-AP (CD147 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11989-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (11989-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of MCF-7 using 11989-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD147 antibody (11989-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of A431 using 11989-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD147 antibody (11989-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

Featured Product
KD/KO Validated

CD147 Polyclonal antibody

CD147 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11989-1-AP

Publications(36)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

5F7, Basigin, BSG, Collagenase stimulatory factor, EMMPRIN

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (36)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, human liver tissue, Jurkat cells, mouse heart tissue, mouse liver tissue, HepG2 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human stomach cancer tissue, human liver cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HeLa cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 22 publications below
IHC	See 10 publications below
IF	See 4 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

11989-1-AP targets CD147 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD147 fusion protein Ag2619 Predict reactive species
Full Name	basigin (Ok blood group)
Calculated Molecular Weight	385 aa, 42 kDa
Observed Molecular Weight	35-58 kDa
GenBank Accession Number	BC009040
Gene Symbol	CD147
Gene ID (NCBI)	682
RRID	AB_2290597
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35613
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).

Protocols

Product Specific Protocols
WB protocol for CD147 antibody 11989-1-AP	Download protocol
IHC protocol for CD147 antibody 11989-1-AP	Download protocol
IF protocol for CD147 antibody 11989-1-AP	Download protocol
IP protocol for CD147 antibody 11989-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover Authors - Michael J Podolsky View Article
human	WB	J Extracell Vesicles Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles Authors - Liqiao Hu View Article
human	WB	J Clin Invest Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders Authors - Huilun Helen Wang View Article
mouse	CoIP	Cell Mol Immunol CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ. Authors - Ruo Chen View Article
mouse	IHC	Acta Pharmacol Sin 124I-labeled anti-CD147 antibody for noninvasive detection of CD147-positive pan-cancers: construction and preclinical studies Authors - Xiao-Kun Ma View Article
human	WB	Elife Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes Authors - Abhishek Jamwal View Article