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BRUNOL6 Polyclonal antibody

BRUNOL6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19078-1-AP

Synonyms

CUG-BP- and ETR-3-like factor 6, CELF-6, CELF6, CELF 6, Bruno-like protein 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BRUNOL6 Polyclonal antibody (19078-1-AP)

    WB analysis using 19078-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BRUNOL6 Polyclonal antibody (19078-1-AP)

    WB analysis of HeLa using 19078-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19078-1-AP (BRUNOL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using BRUNOL6 Polyclonal antibody (19078-1-AP)

    IHC staining of human kidney using 19078-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using BRUNOL6 Polyclonal antibody (19078-1-AP)

    IHC staining of human kidney using 19078-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BRUNOL6 Polyclonal antibody (19078-1-AP)

    IF Staining of HeLa using 19078-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 19078-1-AP (BRUNOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue, pig brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19078-1-AP targets BRUNOL6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRUNOL6 fusion protein Ag4970
Full Name bruno-like 6, RNA binding protein (Drosophila)
Calculated Molecular Weight 481 aa, 50 kDa
Observed Molecular Weight 50 kDa, 39 kDa
GenBank Accession NumberBC030835
Gene Symbol BRUNOL6
Gene ID (NCBI) 60677
RRIDAB_2243662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IHC protocol for BRUNOL6 antibody 19078-1-APDownload protocol
IF protocol for BRUNOL6 antibody 19078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cell Death Dis

Long noncoding RNA LINC01594 inhibits the CELF6-mediated splicing of oncogenic CD44 variants to promote colorectal cancer metastasis

Authors - Bowen Liu
View Article