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  • Featured Product
  • KD/KO Validated

ATRIP Polyclonal antibody

ATRIP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11327-1-AP

Synonyms

AGS1, ATR interacting protein, ATRIP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATRIP Polyclonal antibody (11327-1-AP)

    WB analysis using 11327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATRIP Polyclonal antibody (11327-1-AP)

    WB analysis of HeLa using 11327-1-AP

    WB result of ATRIP antibody (11327-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATRIP transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATRIP Polyclonal antibody (11327-1-AP)

    IP experiment of HeLa using 11327-1-AP

    IP result of anti-ATRIP (IP:11327-1-AP, 4ug; Detection:11327-1-AP 1:5000) with HeLa cells lysate 1200 ug.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, PC-3 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11327-1-AP targets ATRIP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATRIP fusion protein Ag1869
Full Name ATR interacting protein
Calculated Molecular Weight 791 aa, 86 kDa
Observed Molecular Weight 86-90 kDa
GenBank Accession NumberBC014153
Gene Symbol ATRIP
Gene ID (NCBI) 84126
RRIDAB_10837229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATRIP (ATR Interacting Protein) and ATR signalling complex is a central mediator of the replicative stress response (RSR),and mutations in both genes have been reported in Seckel syndrome.

Protocols

Product Specific Protocols
WB protocol for ATRIP antibody 11327-1-APDownload protocol
IP protocol for ATRIP antibody 11327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Biomaterials

A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction.

Authors - Jin Qiu
  • KD Validated
View Article
humanWB

Front Pharmacol

Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways.

Authors - Li-Fei Gu
View Article
mouseWB

Biochem J

Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance

Authors - Jill E Hunter
View Article
mouseWB

Biochem J

Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway

Authors - Jill E Hunter
View Article
humanWB

Cell Insight

ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance

Authors - Rong Luo
View Article