Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
11327-1-AP targets ATRIP in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1869 Product name: Recombinant human ATRIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 464-765 aa of BC014153 Sequence: PEDSVCILEGFSVTALSILQHLVCHSGAVVSLLLSGVGADSAAGEGNRSLVHRLSDGDMTSALRGVADDQGQHPLLKMLLHLLAFSSAATGHLQASVLTQCLKVLVKLAENTSCDFLPRFQCVFQVLPKCLSPETPLPSVLLAVELLSLLADHDQLAPQLCSHSEGCLLLLLYMYITSRPDRVALETQWLQLEQEVVRALTVMLHRQWLTVRRAGGPPRTDQQRRTVRCLRDTVLLLHGLSQKDKLFMMHCVEVLHQFDQVMPGVSMLIRGLPDVTDCEEAALDDLCAAETDVEDPEVECG Predict reactive species |
| Full Name | ATR interacting protein |
| Calculated Molecular Weight | 791 aa, 86 kDa |
| Observed Molecular Weight | 86-90 kDa |
| GenBank Accession Number | BC014153 |
| Gene Symbol | ATRIP |
| Gene ID (NCBI) | 84126 |
| RRID | AB_10837229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXE1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATRIP (ATR Interacting Protein) and ATR signalling complex is a central mediator of the replicative stress response (RSR),and mutations in both genes have been reported in Seckel syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ATRIP antibody 11327-1-AP | Download protocol |
| WB protocol for ATRIP antibody 11327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomaterials A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction.
| ||
Front Pharmacol Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways. | ||
Biochem J Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance | ||
Cell Insight ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance | ||
Oncogene Phospho-TRIM21 orchestrates RPA2 ubiquitination switch to promote homologous recombination and tumor radio/chemo-resistance |
