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  • Featured Product
  • KD/KO Validated

Alpha Actinin Polyclonal antibody

Alpha Actinin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 11313-2-AP

Synonyms

Alpha actinin 1, ACTN1, Alpha-actinin cytoskeletal isoform, ACTN 1, actinin, alpha 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis using 11313-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis of HeLa using 11313-2-AP

    WB result of Alpha Actinin antibody (11313-2-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Actinin transfected HeLa cells.

  • Western Blot (WB) analysis of rat heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis of rat heart using 11313-2-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis of mouse spleen using 11313-2-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IHC staining of human heart using 11313-2-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IHC staining of human heart using 11313-2-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of rat heart using 11313-2-AP

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse heart using 11313-2-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse skeletal muscle using 11313-2-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse skeletal muscle using 11313-2-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse heart using 11313-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of rat heart using 11313-2-AP

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using Alpha Actinin Polyclonal antibody (11313-2-AP)

    FC experiment of A431 using 11313-2-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (11313-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, mouse spleen tissue, rat heart tissue, C6 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat heart tissue, mouse heart tissue, mouse skeletal muscle tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11313-2-AP targets Alpha Actinin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag1859
Full Name actinin, alpha 1
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC015766
Gene Symbol Alpha Actinin
Gene ID (NCBI) 87
RRIDAB_2223815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
WB protocol for Alpha Actinin antibody 11313-2-APDownload protocol
IHC protocol for Alpha Actinin antibody 11313-2-APDownload protocol
IF protocol for Alpha Actinin antibody 11313-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Redox Biol

MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling

Authors - Kai-Na Shi
View Article
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
View Article
humanIF

Adv Healthc Mater

A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.

Authors - Zhi Zheng
View Article
mouseWB

Cell Death Differ

Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.

Authors - Ming Zou
View Article
mouseIF

Transl Res

Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.

Authors - Pengyu Jia
View Article
mouseWB

Theranostics

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Authors - Jing Shi
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Jason (Verified Customer) (10-28-2019)

Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Mouse myocardium
Alpha Actinin Antibody Immunohistochemistry, validation (1/100 dilution) in Mouse myocardium (Cat no:11313-2-AP)
FH

Steeve (Verified Customer) (01-08-2018)

Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1'1000
  • Cell Tissue Type: human umbilical veins