Validation Data Gallery
|Positive WB detected in||mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16215-1-AP targets ACAT1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACAT1 fusion protein Ag9215|
|Full Name||acetyl-Coenzyme A acetyltransferase 1|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||38-45 kDa|
|GenBank accession number||BC010942|
|Gene ID (NCBI)||38|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.
|Product Specific Protocols|
|WB protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IHC protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IF protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|IP protocol for ACAT1 antibody 16215-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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