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ACAT1 Polyclonal antibody

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16215-1-AP

Synonyms

MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase



Tested Applications

Positive WB detected inmouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT1 fusion protein Ag9215
Full Name acetyl-Coenzyme A acetyltransferase 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC010942
Gene Symbol ACAT1
Gene ID (NCBI) 38
RRIDAB_2220210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

Protocols

Product Specific Protocols
WB protocol for ACAT1 antibody 16215-1-APDownload protocol
IHC protocol for ACAT1 antibody 16215-1-APDownload protocol
IF protocol for ACAT1 antibody 16215-1-APDownload protocol
IP protocol for ACAT1 antibody 16215-1-APDownload protocol
FC protocol for ACAT1 antibody 16215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Adv Mater

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Authors - Zhuo Li
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
mouseWB

Sci Adv

Ketogenic diet uncovers differential metabolic plasticity of brain cells

Authors - Tim Düking
mouseWB

Hum Mol Genet

Partial Complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage.

Authors - Peralta Susana S
humanWB

Cell Mol Gastroenterol Hepatol

S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.

Authors - Linqiang Zhang