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  • Featured Product
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ACAT1 Polyclonal antibody

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16215-1-AP

Synonyms

MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of MCF-7 using 16215-1-AP

    WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis using 16215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of mouse heart using 16215-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of rat skeletal muscle using 16215-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    WB analysis of human skeletal muscle using 16215-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IP experiment of mouse heart using 16215-1-AP

    IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human liver using 16215-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human liver using 16215-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human liver using 16215-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon using ACAT1 Polyclonal antibody (16215-1-AP)

    IHC staining of human colon using 16215-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ACAT1 Polyclonal antibody (16215-1-AP)

    IF Staining of HeLa using 16215-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using ACAT1 Polyclonal antibody (16215-1-AP)

    FC experiment of HeLa using 16215-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT1 fusion protein Ag9215
Full Name acetyl-Coenzyme A acetyltransferase 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC010942
Gene Symbol ACAT1
Gene ID (NCBI) 38
RRIDAB_2220210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.

Protocols

Product Specific Protocols
WB protocol for ACAT1 antibody 16215-1-APDownload protocol
IHC protocol for ACAT1 antibody 16215-1-APDownload protocol
IF protocol for ACAT1 antibody 16215-1-APDownload protocol
IP protocol for ACAT1 antibody 16215-1-APDownload protocol
FC protocol for ACAT1 antibody 16215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Adv Mater

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Authors - Zhuo Li
View Article
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
View Article
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
View Article
mouseWB

Sci Adv

Ketogenic diet uncovers differential metabolic plasticity of brain cells

Authors - Tim Düking
View Article
human,mouseWB

Phytomedicine

Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux

Authors - Jianfei Mu
View Article
mouseWB

Hum Mol Genet

Partial Complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage.

Authors - Peralta Susana S
View Article