c-Met (Cytoplasmic) Recombinant antibody, PBS Only
c-Met (Cytoplasmic) Recombinant Antibody for WB, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
2G12
Cat no : 82904-1-PBS
Synonyms
Validation Data Gallery
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
82904-1-PBS targets c-Met (Cytoplasmic) in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | c-Met (Cytoplasmic) fusion protein Ag23140 |
Full Name | met proto-oncogene (hepatocyte growth factor receptor) |
Calculated Molecular Weight | 1390 aa, 155 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | BC130420 |
Gene Symbol | MET |
Gene ID (NCBI) | 4233 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.