LOXL2 Monoclonal antibody, PBS Only

LOXL2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7F1

Cat no : 67139-1-PBS

Synonyms

LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14

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Validation Data Gallery

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  WB analysis of A549 using 67139-1-Ig (same clone as 67139-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67139-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 67139-1-Ig (same clone as 67139-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67139-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 67139-1-Ig (same clone as 67139-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67139-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67139-1-Ig (same clone as 67139-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67139-1-Ig (LOXL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67139-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67139-1-Ig (same clone as 67139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67139-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67139-1-Ig (same clone as 67139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67139-1-Ig (LOXL2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67139-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67139-1-Ig (same clone as 67139-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67139-1-Ig (LOXL2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67139-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67139-1-PBS targets LOXL2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LOXL2 fusion protein Ag28566
• Full Name: lysyl oxidase-like 2
• Calculated Molecular Weight: 774 aa, 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC000594
• Gene Symbol: LOXL2
• Gene ID (NCBI): 4017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).