HRP-conjugated Alpha Tubulin Monoclonal antibody
Alpha Tubulin Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB
Conjugate
HRP Fluorescent Dye
CloneNo.
1E4C11
Cat no : HRP-66031
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 72 publications below |
Product Information
HRP-66031 targets Alpha Tubulin in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein |
| Full Name | tubulin, alpha 1b |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC009314 |
| Gene symbol | TUBA1B |
| Gene ID (NCBI) | 10376 |
| Conjugate | HRP Fluorescent Dye |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
What is the function of alpha-tubulin?
Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport, and are also involved in cell division.
Where is alpha-tubulin expressed?
Alpha-tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.
What are the post-translational modifications of alpha-tubulin?
The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerized microtubule. For example, the first modification to be identified was detyrosination,1 as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyrosination along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs at several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).
Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).
Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).
Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).
Publications
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. | ||
Cell Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. | ||
Immunity Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis. | ||
Nat Immunol Control of CD1d-restricted antigen presentation and inflammation by sphingomyelin. | ||
