Anticorps Polyclonal de lapin anti-SLC7A11/xCT

• Phare
• Validé par KD/KO

SLC7A11/xCT Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

246

Avis (4)

N° de cat : 26864-1-AP

Synonymes

Amino acid transport system xc, CCBR1, Cystine/glutamate transporter, SLC7A11, SLC7A11/xCT, xCT

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Galerie de données de validation

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  WB analysis using 26864-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26864-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26864-1-AP

  IP result of anti-SLC7A11/xCT(IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with A549 cells lysate 960 ug.

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  IP experiment of HepG2 using 26864-1-AP

  IP result of anti-SLC7A11/xCT(IP:26864-1-AP, 4ug; Detection:26864-1-AP 1:800) with HepG2 cells lysate 1720 ug.

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  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26864-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26864-1-AP (SLC7A11/xCT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules BxPC-3, cellules HepG2, cellules U2OS
• Résultats positifs en IP: cellules A549, cellules HepG2
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 223 publications below
• IHC: See 55 publications below
• IF: See 28 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

26864-1-AP cible SLC7A11/xCT dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Chèvre, Humain, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC7A11/xCT Protéine recombinante Ag25431
• Nom complet: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa, 35-40 kDa
• Numéro d’acquisition GenBank: BC012087
• Symbole du gène: SLC7A11
• Identification du gène (NCBI): 23657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer. 26864-1-AP recognizes the 55 kDa and 35-40 kDa protein similar to the paper published (PMID: 32188872, 19156441).

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
human	WB	Nat Immunol; Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.; Authors - Pengchong Li
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
human	WB	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Nat Chem Biol; Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals; Authors - Uladzimir Barayeu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chloe (Verified Customer) (11-01-2023)

Great antibody works pretty well for a western blot with 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast Cancer Cell Lines

SLC7A11/xCT Antibody Western Blot validation (1:1000 dilution) in Breast Cancer Cell Lines (Cat no:26864-1-AP)

FH

James (Verified Customer) (11-18-2022)

Detection of SLC7A11 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

SLC7A11/xCT Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:26864-1-AP)

FH

Melanie (Verified Customer) (05-11-2022)

Tested the antibody in Hek293T cells. Had to dilute the antibody 1:50000, since the signal was to strong. Very clear gang at the calculated MW. Good antibody!

• Applications: Western Blot
• Cell Tissue Type: Hek293T

FH

Amélie (Verified Customer) (05-03-2022)

We used the AB on Bone Marrow derived Macrophages, where Slc7a11 is KO (validate by RT PCR), in Western blot. We did not found a specific staining in the right size.

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: Bone Marrow derived macrophages from WT or KO mice

SLC7A11/xCT Antibody Western Blot validation (1000 dilution) in Bone Marrow derived macrophages from WT or KO mice (Cat no:26864-1-AP)