Anticorps Monoclonal anti-vwf

vwf Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3F9F3

N° de cat : CL594-66682

Synonymes

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF

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Galerie de données de validation

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  IF Staining of human breast cancer using CL594-66682

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 vwf antibody (CL594-66682, Clone: 3F9F3 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL594-66682

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 vwf antibody (CL594-66682, Clone: 3F9F3 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF: tissu de cancer du sein humain,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66682 cible vwf dans les applications de IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: vwf Protéine recombinante Ag25578
• Nom complet: von Willebrand factor
• Symbole du gène: VWF
• Identification du gène (NCBI): 7450
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).