• Aperçu
  • Applications testées
  • Informations sur le produit
  • Publications (7)
  • Protocole
  • Avis (1)

Anticorps Monoclonal anti-Phospho-TDP43 (Ser403/404)

Phospho-TDP43 (Ser403/404) Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

6B11B12

N° de cat : 66079-1-Ig

Synonymes

ALS10, Phospho-TDP43 (Ser403/404), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43



Applications testées

Résultats positifs en WBcellules HEK-293, cellules Jurkat, cellules K-562, cellules RAW 264.7, tissu cérébral humain fœtal
Résultats positifs en cytométriecellules Jurkat,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66079-1-Ig cible Phospho-TDP43 (Ser403/404) dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, poisson-zèbre
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet TAR DNA binding protein
Masse moléculaire calculée 43 kDa
Poids moléculaire observé 25 kDa
Numéro d’acquisition GenBankNM_007375
Symbole du gène TARDBP
Identification du gène (NCBI) 23435
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Protocole

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser403/404) antibody 66079-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
human,zebrafishIHC,IF

Nat Neurosci

TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury

Authors - Alessandro Zambusi
humanIHC

Acta Neuropathol Commun

Influence of APOE genotype in primary age-related tauopathy.

Authors - Andrew C Robinson
humanWB

Eur J Med Chem

Targeting nuclear protein TDP-43 by cell division cycle kinase 7 inhibitors: A new therapeutic approach for amyotrophic lateral sclerosis.

Authors - Elisa Rojas-Prats
humanIF

Biochemistry

Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.

Authors - Janice S W Ng
humanWB

PLoS One

Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects.

Authors - Jer-Cherng Chang
humanWB

Methods Mol Biol

Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells

Authors - Lara A Gruijs da Silva

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by Phospho-TDP43 (Ser403/404) Monoclonal antibody at dilution of 1:100 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: NSC-34 cells

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