Anticorps Polyclonal de lapin anti-pan-keratin

pan-keratin Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-26411

Synonymes

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, pan-keratin, Type II keratin Kb5

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Galerie de données de validation

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  IF Staining of human colon cancer using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

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  IF Staining of human colon cancer using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Cyan) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

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  IF Staining of HeLa using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

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  IF Staining of human lung cancer using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

• 

  IF Staining of human colon cancer using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

• 

  IF Staining of human colon cancer using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

• 

  IF Staining of human lung cancer using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL488-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200.

• 

  FC experiment of A431 using CL488-26411

  1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human pan-keratin (CL488-26411) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules HeLa, tissu de cancer du côlon humain, tissu de cancer du poumon humain
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-26411 cible pan-keratin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: pan-keratin Protéine recombinante Ag24184
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: KRT5
• Identification du gène (NCBI): 3852
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C