Anticorps Monoclonal anti-NF-κB p65

• Phare
• Validé par KD/KO

NF-κB p65 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (3)

Applications

WB, RIP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

186

CloneNo.

1B12D11

Avis (2)

N° de cat : 66535-1-Ig

Synonymes

NF-kB, NFKB3, NF-κB, NF-κB p65, NF-κB3, p65, RELA, Transcription factor p65

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Galerie de données de validation

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  WB analysis of HEK-293 using 66535-1-Ig

  WB result of p65; RELA antibody (66535-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p65; RELA transfected HEK-293 cells.

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  WB analysis using 66535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66535-1-Ig (NF-κB p65 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66535-1-Ig (NF-κB p65 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of human breast cancer using 66535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig (p65; RELA antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66535-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig (p65; RELA antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66535-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF-κB p65 antibody (66535-1-Ig, Clone: 1B12D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules MOLT-4, cellules NIH/3T3, cellules Raji
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 168 publications below
• IHC: See 15 publications below
• IF: See 28 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

66535-1-Ig cible NF-κB p65 dans les applications de WB, RIP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, porc, poulet
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NF-κB p65 Protéine recombinante Ag1199
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC011603
• Symbole du gène: RELA
• Identification du gène (NCBI): 5970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Publications

Species	Application	Title
	WB	Cell Rep; METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling; Authors - Shuhua Luo
	WB	Theranostics; Prussian blue nanozyme-mediated nanoscavenger ameliorates acute pancreatitis via inhibiting TLRs/NF-κB signaling pathway.; Authors - Xue Xie
	WB	Clin Transl Med; PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer.; Authors - Daiwei Wan
	WB	Phytomedicine; Ginsenoside Rb1 protects hippocampal neurons in depressed rats based on mitophagy-regulated astrocytic pyroptosis; Authors - Yannan Li
human	WB	Br J Pharmacol; Neutrophil Elastase Promotes Neointimal Hyperplasia by Targeting TLR4-NFкB Signalling.; Authors - Mei Yang
	WB,IHC	Biomed Pharmacother; Baicalin ameliorates multidrug-resistant Pseudomonas aeruginosa induced pulmonary inflammation in rat via arginine biosynthesis; Authors - Lei Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (04-19-2023)

Lowest membrane part has NF-kB p65 in U2OS cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

NF-κB p65 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:66535-1-Ig)

FH

Federica (Verified Customer) (01-16-2023)

The band comes out well even when low total protein concentration is loaded (15 ug).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Dendritic cells