Anticorps Monoclonal anti-p21

p21 Monoclonal Antibody for IF, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

souris et plus (2)

Applications

IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3C7D3

N° de cat : 67362-1-Ig

Synonymes

CAP20, CDKI, Cdkn1, Cdkn1a, CIP1, mda6, P21, p21Cip1, p21WAF, SDI1, Waf1

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Galerie de données de validation

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  IF Staining of NIH/3T3 using 67362-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IF: cellules NIH/3T3,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

67362-1-Ig cible p21 dans les applications de IF, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p21 Protéine recombinante Ag28394
• Nom complet: cyclin-dependent kinase inhibitor 1A (P21)
• Masse moléculaire calculée: 18 kDa
• Numéro d’acquisition GenBank: NM_001111099
• Symbole du gène: Cdkn1a
• Identification du gène (NCBI): 12575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

Publications

Species	Application	Title
human,mouse		Front Pharmacol; Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease.; Authors - Xiaoyu Qi
mouse		Life Sci; N6-methyladenosine modification on Hmbox1 is related to telomere dysfunction in DEHP-induced male reproductive injury; Authors - Xiaofang Zhu
canine	IF	Cell Prolif; TGF-β-induced PI3K/AKT/mTOR pathway controls myofibroblast differentiation and secretory phenotype of valvular interstitial cells through the modulation of cellular senescence in a naturally occurring in vitro canine model of myxomatous mitral valve disease; Authors - Qiyu Tang
mouse	IF	Heliyon; Mitochondrial citrate accumulation triggers senescence of alveolar epithelial cells contributing to pulmonary fibrosis in mice; Authors - Jie-Ru Hong