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Anticorps Monoclonal anti-Gamma Tubulin

Gamma Tubulin Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

canin, Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

3F9H8

N° de cat : CL594-66320

Synonymes

Gamma 1 tubulin, Gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin gamma 1 chain, tubulin, gamma 1

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite®594-conjugated Gamma Tubulin Monoclonal a (CL594-66320)

    IF Staining of MDCK using CL594-66320

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CL594-66320 (gamma tubulin antibody) at dilution of 1:50.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Gamma Tubulin Monoclonal a (CL594-66320)

    FC experiment of HeLa using CL594-66320

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Gamma Tubulin (CL594-66320, Clone:3F9H8) (red), or 0.8 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules MDCK,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66320 cible Gamma Tubulin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons canin, Humain, rat, souris

Réactivité canin, Humain, rat, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène Gamma Tubulin Protéine recombinante Ag23913
Nom complet tubulin, gamma 1
Masse moléculaire calculée 51 kDa
Poids moléculaire observé 48-55 kDa
Numéro d’acquisition GenBankBC000619
Symbole du gène TUBG1
Identification du gène (NCBI) 7283
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Protocole

Product Specific Protocols
IF protocol for CL594 Gamma Tubulin antibody CL594-66320Download protocol
Standard Protocols
Click here to view our Standard Protocols