Anticorps Monoclonal anti-cIAP1

cIAP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1H3F1

Avis (1)

N° de cat : 66626-1-Ig

Synonymes

API1, BIRC2, C IAP1, cIAP1, Hiap 2, HIAP2, IAP 2, IAP homolog B, IAP2, MIHB, RING finger protein 48, RNF48

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Galerie de données de validation

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  WB analysis using 66626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66626-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66626-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66626-1-Ig (cIAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSCT6, cellules Jurkat, tissu cérébral de porc, tissu cérébral de rat
• Résultats positifs en IHC: tissu splénique humain, tissu d'amygdalite humain, tissu de cancer du poumon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

66626-1-Ig cible cIAP1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: cIAP1 Protéine recombinante Ag21398
• Nom complet: baculoviral IAP repeat-containing 2
• Masse moléculaire calculée: 618 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC016174
• Symbole du gène: BIRC2
• Identification du gène (NCBI): 329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).

Publications

Species	Application	Title
human	WB	Cell Death Dis; Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.; Authors - Jingru Huangfu
human	WB	Chem Biol Interact; Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65; Authors - Jingwen Tan
human,mouse	WB,IHC,IF,IP	J Exp Clin Cancer Res; TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein; Authors - Xu Yang
mouse	WB,IF	JACC Basic Transl Sci; Bag3 Regulates Mitochondrial Function and the Inflammasome Through Canonical and Noncanonical Pathways in the Heart; Authors - JuFang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shubham (Verified Customer) (10-04-2019)

Good

• Applications: Immunoprecipitation,
• Cell Tissue Type: AC16

cIAP1 Antibody Immunoprecipitation, validation ( dilution) in AC16 (Cat no:66626-1-Ig)