Anticorps Polyclonal de lapin anti-c-SRC

c-SRC Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris, Hamster

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 25978-1-AP

Synonymes

ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Galerie de données de validation

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  WB analysis of A431 using 25978-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25978-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of CHO using 25978-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25978-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25978-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules CHO, cellules COLO 320, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

25978-1-AP cible c-SRC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris, Hamster

• Réactivité: Humain, souris, Hamster
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: c-SRC Protéine recombinante Ag22415
• Nom complet: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC011566
• Symbole du gène: SRC
• Identification du gène (NCBI): 6714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Publications

Species	Application	Title
human	WB	Life Sci; Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma.; Authors - Lin Zhao
human	WB	Oncotarget; Targeting claudin-4 enhances CDDP-chemosensitivity in gastric cancer.; Authors - Yukiko Nishiguchi