Anticorps Monoclonal anti-Beta Arrestin 1

• Phare
• Validé par KD/KO

Beta Arrestin 1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1H8E4

N° de cat : 67580-1-Ig

Synonymes

ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1

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Galerie de données de validation

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  WB analysis using 67580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67580-1-Ig

  WB result of Beta Arrestin 1 antibody (67580-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Arrestin 1 transfected HepG2 cells.

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  WB analysis of HeLa using 67580-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67580-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 67580-1-Ig

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, plaquettes de sang périphérique humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67580-1-Ig cible Beta Arrestin 1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beta Arrestin 1 Protéine recombinante Ag7504
• Nom complet: arrestin, beta 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC003636
• Symbole du gène: ARRB1
• Identification du gène (NCBI): 408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).