Anticorps Monoclonal anti-Beta Actin

Beta Actin Monoclonal Antibody for IF, WB

Hôte / Isotype

Mouse / IgM

Réactivité testée

canin, Humain, plante, poisson-zèbre, rat, souris

Applications

WB, IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

7D2C10

N° de cat : CL594-60008

Synonymes

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

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Galerie de données de validation

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  WB analysis using CL594-60008

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-60008 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using CL594-60008

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.

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  IF Staining of MDCK using CL594-60008

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa
• Résultats positifs en IF: cellules HeLa, cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60008 cible Beta Actin dans les applications de WB, IF et montre une réactivité avec des échantillons canin, Humain, plante, poisson-zèbre, rat, souris

• Réactivité: canin, Humain, plante, poisson-zèbre, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beta Actin Protéine recombinante Ag0297
• Nom complet: actin, beta
• Masse moléculaire calculée: 375 aa, 42 kDa
• Numéro d’acquisition GenBank: BC002409
• Symbole du gène: ACTB
• Identification du gène (NCBI): 60
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL594,Ex/Em 593 nm/614 nm.