Anticorps Polyclonal de lapin anti-ZCRB1

ZCRB1 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 25629-1-AP

Synonymes

MADP 1, MADP1, RBM36, U11/U12 31K, U11/U12 snRNP 31 kDa protein, ZCCHC19, ZCRB1

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Galerie de données de validation

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  WB analysis of mouse testis using 25629-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 25629-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25629-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25629-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25629-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25629-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25629-1-AP

  Immunofluorescent analysis of HepG2 cells using 25629-1-AP (ZCRB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules Neuro-2a, cellules Raji, cellules SMMC-7721
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

25629-1-AP cible ZCRB1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZCRB1 Protéine recombinante Ag22095
• Nom complet: zinc finger CCHC-type and RNA binding motif 1
• Masse moléculaire calculée: 217 aa, 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC022543
• Symbole du gène: ZCRB1
• Identification du gène (NCBI): 85437
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5.; Authors - Jian Song