Anticorps Monoclonal anti-ZAP70

• Phare
• Validé par KD/KO

ZAP70 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

3B3C6

N° de cat : 60200-2-Ig

Synonymes

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

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Galerie de données de validation

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  WB analysis of Jurkat using 60200-2-Ig

  WB result of ZAP70 antibody (60200-2-Ig, 1:5000) with si-control and si-ZAP70 transfected Jurkat cells..

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  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 60200-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 60200-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60200-2-Ig cible ZAP70 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZAP70 Protéine recombinante Ag7943
• Nom complet: zeta-chain (TCR) associated protein kinase 70kDa
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC039039
• Symbole du gène: ZAP70
• Identification du gène (NCBI): 7535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.