Anticorps Monoclonal anti-YTHDF1 / YTHDF2

YTHDF1 / YTHDF2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2F5H2

N° de cat : 68416-1-Ig

Synonymes

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2



Applications testées

Résultats positifs en WBcellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

68416-1-Ig cible YTHDF1 / YTHDF2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène YTHDF1 / YTHDF2 Protéine recombinante Ag5922
Nom complet YTH domain family, member 2
Masse moléculaire calculée 62 kDa
Poids moléculaire observé63 kDa, 70 kDa
Numéro d’acquisition GenBankBC002559
Symbole du gène YTHDF2
Identification du gène (NCBI) 51441
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.

Protocole

Product Specific Protocols
WB protocol for YTHDF1 / YTHDF2 antibody 68416-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols