Anticorps Monoclonal anti-YAP1

YAP1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

3A7A9

N° de cat : CL594-66900

Synonymes

65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-66900

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66900 (YAP1 antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL594-66900

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 YAP1 antibody (CL594-66900, Clone: 3A7A9 ) at dilution of 1:100.

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Applications testées

• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-66900 cible YAP1 dans les applications de IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: YAP1 Protéine recombinante Ag28194
• Nom complet: Yes-associated protein 1, 65kDa
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC038235
• Symbole du gène: YAP1
• Identification du gène (NCBI): 10413
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65-70 kDa. (PMID: 26695440)

Publications

Species	Application	Title
human	IF	Cell Biosci; CXCL12/CXCR7/β-arrestin1 biased signal promotes epithelial-to-mesenchymal transition of colorectal cancer by repressing miRNAs through YAP1 nuclear translocation; Authors - Mahan Si