- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-YAP1
YAP1 Monoclonal Antibody for IF, IHC, WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugaison
Non conjugué
CloneNo.
3A7A9
N° de cat : 66900-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules NCI-H1299, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HUVEC, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, cellules NIH-3T3 |
| Résultats positifs en IHC | tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de tumeur ovarienne humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 8 publications below |
| WB | See 30 publications below |
| IHC | See 10 publications below |
| IF | See 20 publications below |
| IP | See 1 publications below |
| CoIP | See 3 publications below |
Informations sur le produit
66900-1-Ig cible YAP1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | YAP1 Protéine recombinante Ag28194 |
| Nom complet | Yes-associated protein 1, 65kDa |
| Masse moléculaire calculée | 504 aa, 54 kDa |
| Poids moléculaire observé | 65-70 kDa |
| Numéro d’acquisition GenBank | BC038235 |
| Symbole du gène | YAP1 |
| Identification du gène (NCBI) | 10413 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). Phosphorylation of S381 primes YAP phosphorylation by CK1δ/ɛ, resulting in activation of a phosphodegron, thus generates a binding surface that interacts with a ubiquitin ligase, and leads to degradation by ubiquitination. (PMID: 20048001). The calcualted molecular weight of YAP1is 54 kDa, but phosphorylated YAP1 is about 65-70 kDa. (PMID: 26695440)
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for YAP1 antibody 66900-1-Ig | Download protocol |
| IHC protocol for YAP1 antibody 66900-1-Ig | Download protocol |
| IF protocol for YAP1 antibody 66900-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation | ||
Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.
| ||
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer | ||
Oncogene Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.
| ||
Acta Pharmacol Sin Critical role of PAFR/YAP1 positive feedback loop in cardiac fibrosis.
| ||
Cell Mol Gastroenterol Hepatol Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (11-26-2023) | Worked well at 1:1000 for WB
|
FH Joshua (Verified Customer) (01-29-2019) | Coverslips were fixed with 4%PFA+0.25% Triton-X-100. Primary staining was 1:500, secondary was 1:750 with rockland 488 anti-mouse overnight at 4C.Left panel: Control PANC1 cells.Right panel: PANC1 cells with YAP silencing
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