Anticorps Monoclonal anti-CRM1

CRM1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

2D3B6

N° de cat : 66763-1-Ig

Synonymes

CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1

---

Galerie de données de validation

• 

  WB analysis using 66763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66763-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CRM1 antibody (66763-1-Ig, Clone: 2D3B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66763-1-Ig cible CRM1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CRM1 Protéine recombinante Ag27337
• Nom complet: exportin 1 (CRM1 homolog, yeast)
• Masse moléculaire calculée: 1071 aa, 123 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC032847
• Symbole du gène: XPO1
• Identification du gène (NCBI): 7514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Publications

Species	Application	Title
human,rat	WB	Diabetologia; Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.; Authors - Mu Zhang
	WB	Sci Rep; Anti-tumor efficacy of Selinexor (KPT-330) in gastric cancer is dependent on nuclear accumulation of p53 tumor suppressor.; Authors - Vinod Vijay Subhash
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	Exp Cell Res; NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.; Authors - Jianning Xu
human	IHC	PeerJ; Hub gene associated with prognosis in bladder cancer is a novel therapeutic target; Authors - Dengpan Fang
human	WB	Cell Death Dis; Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells.; Authors - Xiaogang Chen