- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-XBP1S/XBP1U
XBP1S/XBP1U Polyclonal Antibody for FC (Intra)
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris
Applications
FC (Intra)
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
N° de cat : CL488-24168
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en cytométrie | cellules HepG2 |
Dilution recommandée
Application | Dilution |
---|---|
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL488-24168 cible XBP1S/XBP1U dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | XBP1S/XBP1U Protéine recombinante Ag21454 |
Nom complet | X-box binding protein 1 |
Masse moléculaire calculée | 261 aa, 29 kDa |
Poids moléculaire observé | ~32 kDa; ~60 kDa |
Numéro d’acquisition GenBank | BC000938 |
Symbole du gène | XBP1 |
Identification du gène (NCBI) | 7494 |
Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.
Protocole
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 XBP1S/XBP1U antibody CL488-24168 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |