Anticorps Polyclonal de lapin anti-WWP1

WWP1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 28689-1-AP

Synonymes

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

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Galerie de données de validation

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  WB analysis of HepG2 using 28689-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28689-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 28689-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 28689-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28689-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28689-1-AP cible WWP1 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WWP1 Protéine recombinante Ag30161
• Nom complet: WW domain containing E3 ubiquitin protein ligase 1
• Masse moléculaire calculée: 922 aa, 105 kDa
• Poids moléculaire observé: 95-105 kDa
• Numéro d’acquisition GenBank: BC036065
• Symbole du gène: WWP1
• Identification du gène (NCBI): 11059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.