Anticorps Monoclonal anti-WNT2

• Phare
• Validé par KD/KO

WNT2 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

3H7B10

N° de cat : 66656-1-Ig

Synonymes

Int 1 like protein 1, Int 1 related protein, INT1L1, IRP, Protein Wnt 2, WNT2

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Galerie de données de validation

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  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of RAW 264.7 using 66656-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66656-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66656-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-Ig (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 66656-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66656-1-Ig (WNT2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

66656-1-Ig cible WNT2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WNT2 Protéine recombinante Ag25185
• Nom complet: wingless-type MMTV integration site family member 2
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC029854
• Symbole du gène: WNT2
• Identification du gène (NCBI): 7472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

Publications

Species	Application	Title
human	WB,IF	Sci Adv; Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer; Authors - Atish Mohanty ; KD Validated
human	IF	Cell Mol Life Sci; α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway.; Authors - Zewu Zhu
human	WB	J Surg Res; Expression Analysis of ACSL5 and Wnt2B in Human Congenital Pulmonary Airway Malformations.; Authors - Yuan Qu
rat	WB	Int J Endocrinol; Fufang Xueshuantong Improves Diabetic Cardiomyopathy by Regulating the Wnt/β-Catenin Pathway; Authors - Meizhong Peng
human	WB	Int J Oncol; FUT2 promotes the tumorigenicity and metastasis of colorectal cancer cells via the Wnt/β‑catenin pathway; Authors - Peng Liu