Anticorps Polyclonal de lapin anti-WEE2

WEE2 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

N° de cat : 55119-1-AP

Synonymes

WEE1 homolog 2 (S. pombe), Wee1 like protein kinase 1B, Wee1 like protein kinase 2, WEE1B, Wee1B kinase, WEE2

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Galerie de données de validation

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  WB analysis of Jurkat using 55119-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55119-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human ovary using 55119-1-AP

  human ovary tissue were subjected to SDS PAGE followed by western blot with 55119-1-AP (WEE2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu ovarien humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55119-1-AP cible WEE2 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: WEE1 homolog 2 (S. pombe)
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 63-66 kDa
• Numéro d’acquisition GenBank: NM_001105558
• Symbole du gène: WEE2
• Identification du gène (NCBI): 494551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: NaHCO3 0,1 M, glycine 0,1 M, azoture de sodium à 0,02 % et glycérol à 50 %, pH 7,3.
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WEE2, also named as WEE1B, belongs to the protein kinase superfamily. Ser/Thr protein kinase family and WEE1 subfamily. It phosphorylates and inhibits CDK1. WEE2 may act as a negative regulator of entry into mitosis (G2 to M transition). WEE2 is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Taking the long double-stranded RNA-mediated RNA interference approach, down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. Overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.(PMID: 20200212) The antibody is specific to WEE2.