Anticorps Polyclonal de lapin anti-VEGFD

VEGFD Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 26915-1-AP

Synonymes

c Fos induced growth factor, FIGF, VEGF D, VEGFD

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Galerie de données de validation

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  WB analysis using 26915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26915-1-AP (VEGFD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26915-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26915-1-AP (VEGFD antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26915-1-AP (VEGFD antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26915-1-AP (VEGFD antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26915-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26915-1-AP (VEGFD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules MCF-7, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque humain, tissu de cancer de la thyroïde humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

26915-1-AP cible VEGFD dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEGFD Protéine recombinante Ag25552
• Nom complet: c-fos induced growth factor (vascular endothelial growth factor D)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC027948
• Symbole du gène: FIGF
• Identification du gène (NCBI): 2277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VEGFD is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C.

Publications

Species	Application	Title
human	WB,IHC	Gastric Cancer; Cathepsin L promotes angiogenesis by regulating the CDP/Cux/VEGF-D pathway in human gastric cancer.; Authors - Tao Pan
mouse	IHC	Cereb Cortex; Prolonged Consumption of Sweetened Beverages Lastingly Deteriorates Cognitive Functions and Reward Processing in Mice.; Authors - Héloïse Hamelin
rat	IF	FASEB J; VEGF-D-mediated signaling in tendon cells is involved in degenerative processes.; Authors - Herbert Tempfer
mouse	IHC	Ann Transl Med; Clostridium butyricum alleviates dextran sulfate sodium-induced experimental colitis and promotes intestinal lymphatic vessel regeneration in mice.; Authors - Xing Chen
mouse	IHC	BMC Complement Med Ther; Effects of plant-based medicinal food on postoperative recurrence and lung metastasis of gastric cancer regulated by Wnt/β-catenin-EMT signaling pathway and VEGF-C/D-VEGFR-3 cascade in a mouse model; Authors - Lin Tian
mouse,human	WB,IF	Biochim Biophys Acta Mol Basis Dis; WTAP promotes macrophage recruitment and increases VEGF secretion via N6-methyladenosine modification in corneal neovascularization; Authors - Yanhui Bai