Anticorps Monoclonal anti-VEGFA

• Phare
• Validé par KD/KO

VEGFA Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

88

CloneNo.

2E2H9

N° de cat : 66828-1-Ig

Synonymes

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Galerie de données de validation

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  WB analysis of MCF-7 using 66828-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 66828-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 66828-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66828-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66828-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 66828-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66828-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-231 using 66828-1-Ig

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66828-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66828-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human endometrial cancer using 66828-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human endometrial cancer using 66828-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 66828-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 66828-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of MCF-7 using 66828-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human VEGFA (66828-1-Ig, Clone:2E2H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules HuH-7, cellules HUVEC, cellules MDA-MB-231, cellules NCI-H1299, cellules SH-SY5Y, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 76 publications below
• IHC: See 13 publications below
• IF: See 11 publications below
• ELISA: See 1 publications below

Informations sur le produit

66828-1-Ig cible VEGFA dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VEGFA Protéine recombinante Ag13500
• Nom complet: vascular endothelial growth factor A
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC065522
• Symbole du gène: VEGFA
• Identification du gène (NCBI): 7422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

Publications

Species	Application	Title
	WB	Adv Sci (Weinh); Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution; Authors - Taifeng Zhou
pig	WB	Redox Biol; Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway.; Authors - Chengjun Hu ; KD Validated
human	WB	J Transl Med; KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation; Authors - Yujie Jia
human	WB	Oxid Med Cell Longev; Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.; Authors - Ziwei Yang
human	WB	Mol Ther Nucleic Acids; circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis.; Authors - Ruicheng Yang
human	WB	Bioengineered; LEM domain containing 1 (LEMD1) transcriptionally activated by SRY-related high-mobility-group box 4 (SOX4) accelerates the progression of colon cancer by upregulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway; Authors - Ding Li