Anticorps Polyclonal de lapin anti-VEGFA

VEGFA Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 26381-1-AP

Synonymes

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Galerie de données de validation

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  WB analysis using 26381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26381-1-AP (VEGFA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of NIH/3T3 using 26381-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human VEGFA (26381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

26381-1-AP cible VEGFA dans les applications de WB, IHC, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEGFA Protéine recombinante Ag24740
• Nom complet: vascular endothelial growth factor A
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC065522
• Symbole du gène: VEGFA
• Identification du gène (NCBI): 7422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

Publications

Species	Application	Title
human	WB	Antioxidants (Basel); Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury; Authors - Corry D Bondi
human	WB,CoIP, IHC	J Cell Mol Med; Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis.; Authors - Xiaofei Wang
human	WB,IHC	Oncol Lett; Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer.; Authors - Yu Miao
human	WB	Cell Death Discov; Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer.; Authors - Longyang Jin