Anticorps Monoclonal anti-VCP

• Phare
• Validé par KD/KO

VCP Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2A4B10

N° de cat : 60316-1-Ig

Synonymes

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

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Galerie de données de validation

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  WB analysis of U2OS using 60316-1-Ig

  U2OS (WT and VCP KO) lysates prepared with RIPA buffer, 10μg protein loaded. 60316-1-Ig incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 60316-1-Ig

  WB result of VCP antibody (60316-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VCP transfected HeLa cells.

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  WB analysis of RAW 264.7 using 60316-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 60316-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60316-1-Ig (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 60316-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60316-1-Ig (VCP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of RAW 264.7 using 60316-1-Ig

  Immunofluorescent analysis of RAW 264.7 cells using 60316-1-Ig (VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  IF Staining of HeLa using 60316-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (60316-1-Ig, Clone: 2A4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of SH-SY5Y using 60316-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60316-1-Ig(VCP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HL-60 using 60316-1-Ig

  1X10^6 HL-60 cells were intracellularly stained with 0.4 ug Anti-Human VCP (60316-1-Ig, Clone:2A4B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules RAW 264.7, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules HL-60,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

60316-1-Ig cible VCP dans les applications de WB, IHC, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VCP Protéine recombinante Ag1002
• Nom complet: valosin-containing protein
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC007562
• Symbole du gène: VCP
• Identification du gène (NCBI): 7415
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Publications

Species	Application	Title
human	WB,IF	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič ; KD Validated
human	WB	Acta Pharmacol Sin; Gossypol, a novel modulator of VCP, induces autophagic degradation of mutant huntingtin by promoting the formation of VCP/p97-LC3-mHTT complex.; Authors - Xiao-Jing Li
human	WB,ChIP	Biochim Biophys Acta Gene Regul Mech; GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1; Authors - Luciana L Almada
human	WB,IF	Research (Wash D C); SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage; Authors - Fengli Wang