Anticorps Polyclonal de lapin anti-Uteroglobin/CC10

Uteroglobin/CC10 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 26909-1-AP

Synonymes

CC10, CC16, CCPBP, CCSP, SCGB1A1, UGB, UP 1, UP1, Urinary protein 1, Urine protein 1, Uteroglobin, Uteroglobin/CC10

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Galerie de données de validation

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  WB analysis of rat lung using 26909-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 26909-1-AP (Uteroglobin Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 26909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 26909-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26909-1-AP (Uteroglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A549 using 26909-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (26909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de rat
• Résultats positifs en IHC: tissu pulmonaire humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549
• Résultats positifs en cytométrie: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

26909-1-AP cible Uteroglobin/CC10 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Uteroglobin/CC10 Protéine recombinante Ag25530
• Nom complet: secretoglobin, family 1A, member 1 (uteroglobin)
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 9 kDa
• Numéro d’acquisition GenBank: BC004481
• Symbole du gène: SCGB1A1
• Identification du gène (NCBI): 7356
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Publications

Species	Application	Title
mouse	IF	J Immunol; Regulation of MHC Class I Expression in Lung Epithelial Cells during Inflammation.; Authors - Justine Mathé
rat	IHC	Front Pediatr; Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.; Authors - Jun Wang
human	FC	Front Cell Infect Microbiol; BPIFA1 is a secreted biomarker of differentiating human airway epithelium; Authors - Clarissa Clifton