Anticorps Polyclonal de lapin anti-USP9X

• Phare
• Validé par KD/KO

USP9X Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 55054-1-AP

Synonymes

Deubiquitinating enzyme FAF X, DFFRX, FAF, FAM, Fat facets in mammals, hFAM, Ubiquitin thiolesterase FAF X, USP9, USP9X

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Galerie de données de validation

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  WB analysis using 55054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55054-1-AP (USP9X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55054-1-AP (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55054-1-AP

  IP result of anti-USP9X(IP:55054-1-AP, 4ug; Detection:55054-1-AP 1:1000) with HeLa cells lysate 1280 ug.

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  IP experiment of HeLa using 55054-1-AP

  IP result of anti-USP9X(IP:55054-1-AP, 4ug; Detection:55054-1-AP 1:4000) with HeLa cells lysate 2000 ug.

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  IHC staining of human pancreas cancer using 55054-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55054-1-AP (USP9X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 55054-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55054-1-AP (USP9X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55054-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP9X antibody (55054-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 5 publications below
• CoIP: See 3 publications below

Informations sur le produit

55054-1-AP cible USP9X dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ubiquitin specific peptidase 9, X-linked
• Masse moléculaire calculée: 292 kDa
• Poids moléculaire observé: 260-290 kDa
• Numéro d’acquisition GenBank: NM_001039590
• Symbole du gène: USP9X
• Identification du gène (NCBI): 8239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB	Nat Commun; Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.; Authors - Athea Vichas ; KO Validated
human	IF	Nat Commun; Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.; Authors - Michael Dietachmayr
human	WB,IF,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li ; KO Validated
human	WB	Cell Death Differ; USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.; Authors - Xiaohua Jie
human	IHC	Cancer Res; Deubiquitinating Enzyme USP9X Suppresses Tumor Growth via LATS kinase and Core Components of the Hippo pathway.; Authors - Aleksandra Toloczko