Anticorps Monoclonal anti-USP36

• Phare
• Validé par KD/KO

USP36 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1A1B3

Avis (2)

N° de cat : 68165-1-Ig

Synonymes

Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36

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Galerie de données de validation

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  WB analysis using 68165-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68165-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68165-1-Ig

  WB result of USP36 antibody (68165-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP36 transfected HeLa cells.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68165-1-Ig cible USP36 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: USP36 Protéine recombinante Ag6426
• Nom complet: ubiquitin specific peptidase 36
• Masse moléculaire calculée: 1123 aa, 123 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC038983
• Symbole du gène: USP36
• Identification du gène (NCBI): 57602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chrysa (Verified Customer) (02-01-2023)

Tested in HeLa cells in WB with control si and siRNA against USP36

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

USP36 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:68165-1-Ig)

FH

Chrysa (Verified Customer) (01-17-2023)

Unfortunately, due to couriers mistake the sample stayed 2 days at RT. I tried the antibody 3 times : 1/5000, 1:500 and 1/100 in 5%TBS-milk O/N at 4C in total lysates from HeLa cells (30μg) and didn't work (image uploaded, all the other antibodies worked in the same membrane e.g. anti-tubulin, anti-exosc10, anti-senp3). In 1/100 dilution I got non specific bands. Is it possible that the sample got degraded or is there anything I could do to optimize it to work?

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000, 1:500
• Cell Tissue Type: HeLa