• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-USP22

USP22 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 55110-1-AP

Synonymes

Deubiquitinating enzyme 22, KIAA1063, Ubiquitin thiolesterase 22, USP22, USP3L



Applications testées

Résultats positifs en WBcellules A549, cellules HepG2
Résultats positifs en IPcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

55110-1-AP cible USP22 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet ubiquitin specific peptidase 22
Masse moléculaire calculée 60 kDa
Poids moléculaire observé 58-60 kDa
Numéro d’acquisition GenBankNM_015276
Symbole du gène USP22
Identification du gène (NCBI) 23326
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

USP22, also named as KIAA1063 and USP3L, belongs to the peptidase C19 family and UBP8 subfamily. It is histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. USP22 catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. It is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. USP22 is required for nuclear receptor-mediated transactivation and cell cycle progression. USP22 has 2 isoforms with molecular mass of 58kDa and 60 kDa. The antibody is specific to USP22.

Protocole

Product Specific Protocols
WB protocol for USP22 antibody 55110-1-APDownload protocol
IHC protocol for USP22 antibody 55110-1-APDownload protocol
IP protocol for USP22 antibody 55110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

PMID: 29732405

Authors - Qingxiang Liu
mouseWB

Nat Commun

ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.

Authors - Francesca Bufalieri
humanWB

Cell Oncol (Dordr)

The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.

Authors - Yizhi Xiao
ratWB,IF

Eur J Pharmacol

USP22 aggravated diabetic renal tubulointerstitial fibrosis progression through deubiquitinating and stabilizing Snail1

Authors - Xilin Zhao
  • KD Validated
humanWB,CoIP

Oncol Rep

Targeting USP22 with miR‑30‑5p to inhibit the hypoxia‑induced expression of PD‑L1 in lung adenocarcinoma cells.

Authors - Xiaoyang Hua
ratWB

Endocrinology

AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells

Authors - Kai-Peng Huang
  • KD Validated