- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-USP22
USP22 Polyclonal Antibody for IP, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (2)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 55110-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules A549, cellules HepG2 |
Résultats positifs en IP | cellules HepG2, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 6 publications below |
WB | See 10 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Informations sur le produit
55110-1-AP cible USP22 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | ubiquitin specific peptidase 22 |
Masse moléculaire calculée | 60 kDa |
Poids moléculaire observé | 58-60 kDa |
Numéro d’acquisition GenBank | NM_015276 |
Symbole du gène | USP22 |
Identification du gène (NCBI) | 23326 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
USP22, also named as KIAA1063 and USP3L, belongs to the peptidase C19 family and UBP8 subfamily. It is histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. USP22 catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. It is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. USP22 is required for nuclear receptor-mediated transactivation and cell cycle progression. USP22 has 2 isoforms with molecular mass of 58kDa and 60 kDa. The antibody is specific to USP22.
Protocole
Product Specific Protocols | |
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WB protocol for USP22 antibody 55110-1-AP | Download protocol |
IHC protocol for USP22 antibody 55110-1-AP | Download protocol |
IP protocol for USP22 antibody 55110-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. | ||
Cell Oncol (Dordr) The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer. | ||
Eur J Pharmacol USP22 aggravated diabetic renal tubulointerstitial fibrosis progression through deubiquitinating and stabilizing Snail1
| ||
Oncol Rep Targeting USP22 with miR‑30‑5p to inhibit the hypoxia‑induced expression of PD‑L1 in lung adenocarcinoma cells. | ||
Endocrinology AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells
|